CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters
نویسندگان
چکیده
منابع مشابه
Targeting Hepatitis B Virus With CRISPR/Cas9
Hepatitis B virus persistence in infected hepatocytes is due to the presence of covalently closed circular DNA (cccDNA), the template for the transcription of viral RNAs. Antiviral therapies with nucleoside analogues inhibit replication of HBV DNA in capsids present in the cytoplasm of infected cells, but do not reduce or destroy nuclear cccDNA. To investigate whether cccDNA derived from infect...
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ژورنال
عنوان ژورنال: Genome Biology
سال: 2018
ISSN: 1474-760X
DOI: 10.1186/s13059-018-1534-x